In later experiments we submerged the hoof or toenail fragments in suspensions of T. Five cycles of subtotal UVC inactivation were carried out and the experiments were performed in triplicate for each cycle. Methods Trichophyton rubrum, T. Discussion In the present study, four dermatophyte species, T. Test of dermatophyte resistance to ultraviolet C irradiation Figure 2 demonstrates the cell inactivation curves of T. Trichophyton rubrum colony outgrowth was first observed from the nonirradiated control on day 3, and from the UVC-irradiated fragment on day 6. Due to limited availability of human nail samples, porcine hoof was used as an ex vivo model for human nail in the present study.
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Ultraviolet C inactivation of dermatophytes: implications for treatment of onychomycosis
Significance of correlation coefficient for polynomial curve was estimated by curvilinear regression analysis. To test the ability of UVC to inactivate dermatophyte suspensions in vitro and to sterilize a novel ex vivo model of nail infection. Topical drug treatment for onychomycosis is not usually successful because the topical drugs are typically unable to penetrate the hyperkeratotic nail plate. Mertin D, Lippold BC. The toenail clippings were cut into circular fragments with 4-mm diameter tissue biopsy punches. This allowed a semiquantitative assay for the inactivation rate based on the start date of colony outgrowth on to SDA plates.
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The penetration of UVC in human nail is determined by the nail optical properties including absorption coefficient, scattering coefficient, and anisotropy factor. Determination of chemical agent optical clearing potential using in vitro human skin. The time delays of colony growth in comparison with the nonirradiated control of the microtome-sliced sections at different depths of a UVC-irradiated hoof culture culture 3 in Table 1 are illustrated in Figure 6. Acknowledgments This work was supported by the U. Three logs of cell inactivation of T. The possibility of the development of dermatophyte resistance to UVC irradiation was investigated by carrying out subtotal UVC inactivation leaving c.
Description:Early events in the invasion of the human nail plate by Trichophyton mentagrophytes. If the mutations lead to a competitive advantage they will spread to the whole microbial population. In addition, the cell densities of the suspensions were counted on a haemocytometer Hausser Scientific, Horsham, PA, U. Mature colonies were then harvested, suspended in phosphate-buffered saline PBS , dispersed to single-cell suspensions using a sonic dismembrator Model ; Fisher Scientific, Norcross, GA, U. UVC irradiation of human skin has been much less studied, but is also known to cause the same kind of damage.